ZIO 301

Name	Indibulin
Synonyms	ZIO 301 Nascapine lindiblin Indibulin 2-[1-(4-Chlorobenzyl)-1H-indol-3-yl]-2-oxo-N-pyridin-4-ylacetamide 2-[1-(4-chlorobenzyl)-1H-indol-3-yl]-2-oxo-N-(pyridin-4-yl)acetamide 1H-Indole-3-acetamide, 1-[(4-chlorophenyl)methyl]-α-oxo-N-4-pyridinyl- 2-[1-[(4-chlorophenyl)methyl]indol-3-yl]-2-oxo-N-pyridin-4-ylacetamide 2-(1-(4-Chlorophenylmethyl)-1H-indol-3-yl)-2-oxo-N-(pyridin-4-yl)acetamide
CAS	204205-90-3
InChI	InChI=1/C22H16ClN3O2/c23-16-7-5-15(6-8-16)13-26-14-19(18-3-1-2-4-20(18)26)21(27)22(28)25-17-9-11-24-12-10-17/h1-12,14H,13H2,(H,24,25,28)

Molecular Formula	C22H16ClN3O2
Molar Mass	389.83
Density	1.31±0.1 g/cm3(Predicted)
pKa	9.38±0.70(Predicted)
Storage Condition	Sealed in dry,Store in freezer, under -20°C
Refractive Index	1.664
In vitro study	Indibulin (300-2100 nM; 48 hours) inhibits the proliferation of MCF-7 cells with an IC50 of 150 nM. Indibulin (300, 600 nM; 48 hours) blockes the cells in the G2/M phase indicating that indibulin blockes the progression of the cell cycle at mitosis. Indibulin (150-600 nM; 24 hours) induces apoptosis in MCF-7 cells. Indibulin (150-600 nM; 48 hours) with 300 and 600 nM generates cleaved fragments of PARP protein the treatment of MCF-7 cells. Cell Proliferation Assay Cell Line: MCF-7 cells Concentration: 300, 600, 900, 1200, 1500, 1800, 2100 nM Incubation Time: 48 hours Result: Inhibited the proliferation of MCF-7 cells with an IC 50 of 150 nM. Cell Cycle Analysis Cell Line: MCF-7 cells Concentration: 300, 600 nM Incubation Time: 48 hours Result: Blocked the cells in the G2/M phase of the cell cycle. Apoptosis Analysis Cell Line: MCF-7 cells Concentration: 150, 300 and 600 nM Incubation Time: 24 hours Result: Induced apoptosis in MCF-7 cells. Western Blot Analysis Cell Line: MCF-7 cells Concentration: 150, 300 and 600 nM Incubation Time: 48 hours Result: Generated cleaved fragments of PARP protein in 300 and 600 nM.